epifluorescence microscope equipped light-emitting diode (led) illumination system  (CoolLED Inc)

Journal: Scientific Reports

Article Title: Synthesis and structure of a new thiazoline-based palladium(II) complex that promotes cytotoxicity and apoptosis of human promyelocytic leukemia HL-60 cells

doi: 10.1038/s41598-020-73488-0

Figure Lengend Snippet: Effects of chemotherapeutics on apoptosis in HL-60 cells. Cells were treated with 11.3 µM cisplatin, 20.7 µM PyTT, 20.7 µM PdPyTT, or the vehicle (DMSO, control) for 24 h. ( A ) Hoechst 33342-stained cells were visualized with epifluorescence microscopy and representative images of each experimental condition are shown. The fraction of apoptotic nuclei was evaluated as indicated in the Materials and Methods section. Yellow arrows indicate apoptotic nuclei (i.e., fragmented or condensed). Both fragmented and condensed nuclei are also shown in greater detail in the magnifications. ( B ) Histograms show percentages of cells with apoptotic nucleus. Values represent means ± S.D. of 6 independent experiments. * P < 0.05 compared to control values. Scale bar: 100 µm.

Article Snippet: Then, at least 100 cells per field were counted at the epifluorescence microscope (Nikon Eclipse TS100 equipped with a LED illumination system CoolLED pE-300 white and a digital camera DS-Qi1Mc) in at least three randomly selected microscopic fields.

Techniques: Control, Staining, Epifluorescence Microscopy